This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Campione-Piccardo, J Articles by Bacchetti, S Search for Related Content PubMed PubMed Citation Articles by Campione-Piccardo, J Articles by Bacchetti, S

 Previous Article  |  Next Article 

J Virol. 1979 August; 31(2): 281-287

Selective assay for herpes simplex viruses expressing thymidine kinase.

ABSTRACT

A technique for selecting herpes simplex viruses expressing the viral thymidine kinase (TK+) from a population of predominantly TK- viruses was developed. This was accomplished by infecting TK- cells and incubating the cultures under a liquid overlay medium containing methotrexate. Since the TK- cells survive in this medium for only a limited period of time, it was necessary to add fresh uninfected TK- cells 48 h after infection. The technique allowed the detection and quantitation of the TK+ virus fraction in mixtures of TK+ and TK- viruses where the TK+ fraction was present in frequencies as low as 10(-5). It was also used to estimate reversion frequencies and to obtain and analyze TK+ revertants from TK- mutant strains of herpes simplex virus type 1.

This article has been cited by other articles:

• Cannon, J. S., Hamzeh, F., Moore, S., Nicholas, J., Ambinder, R. F. (1999). Human Herpesvirus 8-Encoded Thymidine Kinase and Phosphotransferase Homologues Confer Sensitivity to Ganciclovir. J. Virol. 73: 4786-4793 [Abstract] [Full Text]  
• Gaynor, R., Feldman, L., Berk, A. (1985). Transcription of class III genes activated by viral immediate early proteins. Science 230: 447-450 [Abstract]