This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lee, H. H. Articles by Miller, L. K. Search for Related Content PubMed PubMed Citation Articles by Lee, H. H. Articles by Miller, L. K.

Isolation, Complementation, and Initial Characterization of Temperature-Sensitive Mutants of the Baculovirus Autographa californica Nuclear Polyhedrosis Virus

Department of Bacteriology and Biochemistry, University of Idaho, Moscow, Idaho 83843

ABSTRACT

Sixteen temperature-sensitive mutants of Autographa californica nuclear polyhedrosis virus were isolated. Several interesting phenotypes were observed. A large proportion of the mutants were unable to form polyhedral occlusion bodies (polyhedra) at the nonpermissive temperature (32.5°C). At 32.5°C, one mutant formed plaques in which the cells lacked polyhedra. Another mutant type was defective in the production of progeny extracellular nonoccluded virus and produced a "plaque" consisting of only a single cell containing polyhedra at 32.5°C. One mutant was defective in plaque formation, progeny nonoccluded virus formation, and polyhedra formation at 32.5°C. Several mutants produced nonoccluded virus but failed to produce plaques or polyhedra at 32.5°C. Other phenotypes were also distinguished. Complementation analyses, performed by either measuring the increase in extracellular nonoccluded virus formation or by observing polyhedra formation in mixed infections at 32.5°C, indicated the presence of 15 complementation groups. A high frequency of recombination was observed. Four of the mutants were found to be host dependent in their temperature sensitivity for polyhedra formation.

Idaho Agricultural Experiment Station research paper no. 7952.

This article has been cited by other articles:

• Kamita, S. G., Maeda, S., Hammock, B. D. (2003). High-Frequency Homologous Recombination between Baculoviruses Involves DNA Replication. J. Virol. 77: 13053-13061 [Abstract] [Full Text]  
• Miller, L. K., Lingg, A. J., Bulla, L. A. Jr. (1983). Bacterial, Viral, and Fungal Insecticides. Science 219: 715-721 [Abstract]