This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Simonsen, C C Articles by Summers, D F Search for Related Content PubMed PubMed Citation Articles by Simonsen, C C Articles by Summers, D F

 Previous Article  |  Next Article 

J Virol. 1979 July; 31(1): 124-132

RNA synthesis of vesicular stomatitis virus-infected cells: in vivo regulation of replication.

ABSTRACT

Pulse-labeling of vesicular stomatitis virus-infected HeLa and BHK cells with [3H]uridine throughout the infectious cycle demonstrated two peaks of uridine incorporation into virus-specific RNA molecules. By separating total RNA synthesis into replication and transcription products, we showed that replication occurs over a shorter period of time in one peak synthesis. The biphasic nature of uridine incorporation is in part due to a general membrane phenomenon of reduced metabolite transport during vesicular stomatis virus infection and in part due to the apparent uncoupling of replication and transcription. A change in the ratio of newly synthesized plus and minus strands of the genome length (42S) RNA was found as the infection proceeded. Early in the infection, plus-stranded 42S RNA comprised 40% of the total genome length RNA synthesis, whereas late in infection, only 15 to 20% of the 42S RNA synthesized was complementary to the virion minus strand. Our data suggest that the rate of synthesis of plus-stranded 42S RNA was constant throughout the infection. The rate of virus release was determined by monitoring the uptake of [3H]uridine into released virus particles. Virus maturation and release are closely associated with the assembly of 42S RNA-containing nucleocapsids.

This article has been cited by other articles:

• Whelan, S. P. J., Wertz, G. W. (1999). Regulation of RNA Synthesis by the Genomic Termini of Vesicular Stomatitis Virus: Identification of Distinct Sequences Essential for Transcription but Not Replication. J. Virol. 73: 297-306 [Abstract] [Full Text]  
• Li, T., Pattnaik, A. K. (1999). Overlapping Signals for Transcription and Replication at the 3' Terminus of the Vesicular Stomatitis Virus Genome. J. Virol. 73: 444-452 [Abstract] [Full Text]