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J Virol. 1969 March; 3(3): 318-325
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Characterization of Influenza Virus Ribonucleic Acid Duplex Produced by Annealing In Vitro

Department of Medical Microbiology and Immunology, School of Medicine, University of California, Los Angeles, California 90024

ABSTRACT

A ribonuclease-resistant ribonucleic acid (RNA) with a sedimentation coefficient of 12S was obtained by self-annealing influenza virus-specific RNA isolated from infected cells. It had the properties of double-stranded RNA. (i) Sedimentation behavior in sucrose gradient was independent of salt concentration. (ii) Thermal transition profile was sharp; the melting temperature is 83 C in 0.1 SSC (0.15 M NaCl plus 0.015 M sodium citrate) and 98 C in SSC. (iii) Buoyant density in cesium sulfate was 1.58 g/cm³ compared to 1.64 g/cm³ for single-stranded RNA. (iv) It gave rise to single-stranded RNA after denaturation. (v) The 12S RNA duplex contained both plus and minus strands of influenza virus. Labeled plus strands could be displaced by extraneous cold plus strands and extraneous ³²P-labeled plus strands could be incorporated into duplex after denaturation and reannealing.