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J Virol. 1969 February; 3(2): 210-216
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Poorly Lytic Bacteriophage from Dactylosporangium thailandensis (Actinomycetales)

Institute of Microbiology, Rutgers-The State University, New Brunswick, New Jersey 08903

ABSTRACT

Dactylosporangiophage A1 has a polygonal head (75 nm) with spherical capsomeres (3 nm) and a noncontractile tail (200 by 10 nm) with cross-striations which is terminated with at least three prongs which are used for attachment. It contains double-stranded deoxyribonucleic acid and produces very little lysis. Intracellular phage multiplication leads to the formation of crystalline aggregates of apparently complete virions. Plaques are formed only on certain substrains of Dactylosporangium thailandensis L1 and are always small (0.5 mm or less). They are clear on some substrains and turbid on others. Formation of plaques occurs only on one medium, Czapek agar with 0.2 to 0.4% yeast extract, 0.2% peptone, or a defined mixture of amino acids. Over 100 strains of bacteria, mainly actinomycetes, were screened in a futile attempt to find an indicator strain which is not a substrain of L1. The Dactylosporangium-phage system studied is considered to be a semiresistant carrier state.

¹ Present address: Department of Microbiology, Temple University School of Medicine, Philadelphia, Pa. 19104.