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J Virol. 1969 February; 3(2): 187-197
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Electron Microscopy of Measles Virus Replication

Masuyo Nakai1 and David T. Imagawa

Department of Pediatrics, UCLA School of Medicine, Harbor General Hospital, Torrance, California 90509
Department of Medical Microbiology and Immunology, UCLA School of Medicine, Harbor General Hospital, Torrance, California 90509

ABSTRACT

Replication of measles virus in HeLa cells was examined by electron microscopy with ultrathin sectioning and phosphotungstic acid negative staining methods. The cytoplasmic inclusion bodies consisted of masses of helical nucleocapsid which was similar in structure to the nucleocapsid found in measles virions. The cytoplasmic helical nucleocapsid appeared to align near the HeLa cell membrane, and the membrane differentiated into the internal membrane of the viral envelope and the outer layer of the short projections. The viral particles were released by a budding process involving incorporation into the viral envelope of membrane which was contiguous to but morphologically altered from the membrane of the HeLa cells. The intranuclear inclusion bodies were composed of tubular structures similar to those found in the cytoplasmic inclusion bodies. These structures aggregated to crystalline arrangement. The relationship between nuclear inclusion body and replication of measles virus was not clear.

FOOTNOTES

1 Present address: Central Laboratory, Osaka Medical College, Osaka, Takatsuki, Japan.

J Virol. 1969 February; 3(2): 187-197
Copyright © 1969 American Society for Microbiology. All Rights Reserved.



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