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J Virol. 1969 February; 3(2): 181-186
Copyright © 1969 American Society for Microbiology. All Rights Reserved.

Bacteriophage Release in a Lysogenic Strain of Agrobacterium tumefaciens¹

Department of Microbiology, The Pennsylvania State University, University Park, Pennsylvania 16802

ABSTRACT

Bacteriophage release in a lysogenic strain of Agrobacterium tumefaciens V-1 is temperature-sensitive. At 25 C and 30 C, phage was released in a ratio of 1 plaque-forming unit per 100 bacteria; at 35 C, although bacterial growth was not inhibited, phage release was suppressed. Phage synthesis was induced by heat shock, 42 C for 30 min, ultraviolet irradiation, and mitomycin C. Induction by ultraviolet light was unusual—an immediate rise in phage titer followed irradiation. A large increase occurred after a 90-min latent period. The lysogenic strain was cured of the phage by incubation at 37 C, and the cured strain produced plant tumors.

¹ Authorized for publication as paper no. 3280 in the journal series of the Pennsylvania Agricultural Experiment Station.