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J Virol. 1979 March; 29(3): 990-996

Biological activity of polyoma viral DNA in mice and hamsters.

ABSTRACT

The biological activity of polyoma viral DNA was evaluated in mice and hamsters. Viral DNA administered parenterally is about 4 to 5 logs less efficient than polyoma virions in establishing infection in mice. Supercoiled viral DNA was infectious for mice after parenteral administration, giving mean infective doses of 10(-3) to 10(-4) microgram. However, animals fed microgram quantities of polyoma DNA I did not become infected. Linearization of viral DNA with R.EcoRI or R.BamHI, which are single-cut enzymes cleaving in the early and late regions of the genome, respectively, reduced the infectivity for mice approximately fivefold. Approximately 10% of newborn hamsters inoculated intraperitoneally with polyoma DNA I developed tumors. In contrast, the same amount of viral DNA which had been cleaved in the early region with R.EcoRI induced tumors in 50% of inoculated hamsters.

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• Israel, M., Chan, H., Martin, M., Rowe, W. (1979). Molecular cloning of polyoma virus DNA in Escherichia coli: oncogenicity testing in hamsters. Science 205: 1140-1142 [Abstract]