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J Virol. 1979 March; 29(3): 1044-1055

Physical Map of the DNA Genome of Autographa californica Nuclear Polyhedrosis Virus

Department of Bacteriology and Biochemistry, The University of Idaho, Moscow, Idaho 83843

ABSTRACT

A physical map of the 88 x 10⁶ dalton, circular DNA genome of Autographa californica nuclear polyhedrosis virus was constructed. The complete order of BamHI and XmaI restriction enzyme sites was determined. The EcoRI and HindIII fragments were partially ordered, and their general locations, relative to the BamHI and XmaI maps, were determined. Alterations in the restriction endonuclease fragment patterns of natural genotypic variants of A. californica nuclear polyhedrosis virus, including Trichoplusia ni MEV nuclear polyhedrosis virus, were located on the physical map. Alterations were found throughout the A. californica nuclear polyhedrosis virus DNA genome.

Research paper no. 78514, published with the approval of the Idaho Agricultural Experiment Station.

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