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J Virol. 1978 December; 28(3): 851-864

Physical mapping of BK virus DNA with SacI, MboII, and AluI restriction endonucleases.

R C Yang and R Wu

ABSTRACT

A new restriction endonuclease, SacI from Streptomyces achromogenes cleaves BK virus (strain MM) DNA into 3 fragments, whereas MboII from Moraxella bovis and AluI from Arthrobacter luteus give 22 and 30 fragments, respectively. All these specific DNA fragments were ordered and mapped on the viral genome by two methods first, by the reciprocal digestion method using uniformly 32P-labeled DNA; and second, by the partial digestion technique using the single-end 32P-labeled DNA. This study, together with those reported earlier, defined the location of 90 cleavage sites on the BK virus DNA.

J Virol. 1978 December; 28(3): 851-864




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Copyright © 1978 by the American Society for Microbiology. All rights reserved.