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J Virol. 1978 July; 27(1): 255-257

Dextran sulfates as a contaminant of DNA extracted from concentrated viruses and as an inhibitor of DNA polymerases.

R A Hitzeman, A M Hanel and A R Price

ABSTRACT

Dextran sulfate is commonly used with polyethylene glycol to concentrate viruses before extraction of their DNA. However, dextran slulfate then easily contaminated such DNA and acted as a potent inhibitor of DNA polymerases from Bacillus subtilis (III), phage PBS2, and phage T4. Dextran sulfate only weakly inhibited Micrococcus luteus and Escherichia coli DNA polymerase I preparations.

J Virol. 1978 July; 27(1): 255-257






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Copyright © 1978 by the American Society for Microbiology. All rights reserved.