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J Virol. 1978 May; 26(2): 536-539

Production of "rapid-harvest" Moloney murine leukemia virus by continuous cell culture on synthetic capillaries.

P L Ratner, M L Cleary and E James

ABSTRACT

Moloney murine leukemia virus was harvested automatically within 60 min of release from chronically infected NIH/3T3 cells (clone 1) cultured on bundles of synthetic capillaries. Production of virus as measured by a determination of reverse transcriptase activity and by the XC syncytia assay demonstrated that highly infectious "rapid-harvest" virus was recovered from NIH/3T3 cells (clone 1) grown for periods of up to 10 days.

J Virol. 1978 May; 26(2): 536-539






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