This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lowy, D R Articles by Scolnick, E M Search for Related Content PubMed PubMed Citation Articles by Lowy, D R Articles by Scolnick, E M

Helper-independent transformation by unintegrated Harvey sarcoma virus DNA.

ABSTRACT

We have studied the unintegrated infectious DNA of Harvey sarcoma virus (Ha-SV) and Moloney leukemia virus (Mo-MuLV). The source of infectious viral DNA was the Hirt supernatant fraction from cells acutely infected with Ha-SV and Mo-MuLV. To obtain a direct quantitative assay for infectious viral DNA, recipient mouse cells were first exposed to calcium phosphate-precipitated viral DNA and then treated with dimethyl sulfoxide. Infectivity was monitored by focus formation for Ha-SV and XC plaque formation for Mo-MuLV. The viral DNA titration pattern followed single-hit kinetics for both foci and plaques, indicating that a single molecule carried information for each function. Focus-forming and plaque-forming activity were present in different molecules, since these two biological activities could be separated from each other by agarose gel electrophoresis. The focus-forming molecule was linear DNA with a molecular weight of about 4 x 10(6) daltons. The focus-forming activity of the viral DNA was sensitive to EcoRI and resistant to XhoI restriction endonucleases, whereas the plaque-forming activity was resistant to EcoRI and sensitive to XhoI. The generation of helper-independent foci indicates that Ha-SV DNA can transform mouse cells in the absence of helper virus or its proteins.

This article has been cited by other articles:

• Sweasy, J. B., Lang, T., Starcevic, D., Sun, K.-W., Lai, C.-C., DiMaio, D., Dalal, S. (2005). Expression of DNA polymerase {beta} cancer-associated variants in mouse cells results in cellular transformation. Proc. Natl. Acad. Sci. USA 102: 14350-14355 [Abstract] [Full Text]  
• Fotiadou, P., Henegariu, O., Sweasy, J. B. (2004). DNA Polymerase {beta} Interacts with TRF2 and Induces Telomere Dysfunction in a Murine Mammary Cell Line. Cancer Res. 64: 3830-3837 [Abstract] [Full Text]  
• Ozbun, M. A. (2002). Human Papillomavirus Type 31b Infection of Human Keratinocytes and the Onset of Early Transcription. J. Virol. 76: 11291-11300 [Abstract] [Full Text]  
• Li, X., Kan, H.-Y., Lavrentiadou, S., Krieger, M., Zannis, V. (2002). Reconstituted Discoidal ApoE-Phospholipid Particles Are Ligands for the Scavenger Receptor BI. THE AMINO-TERMINAL 1-165 DOMAIN OF ApoE SUFFICES FOR RECEPTOR BINDING. J. Biol. Chem. 277: 21149-21157 [Abstract] [Full Text]  
• Redpath, S., Angulo, A., Gascoigne, N. R. J., Ghazal, P. (1999). Murine Cytomegalovirus Infection Down-Regulates MHC Class II Expression on Macrophages by Induction of IL-10. J. Immunol. 162: 6701-6707 [Abstract] [Full Text]  
• Chang, J., Yang, S.-H., Cho, Y.-G., Hwang, S. B., Hahn, Y. S., Sung, Y. C. (1998). Hepatitis C Virus Core from Two Different Genotypes Has an Oncogenic Potential but Is Not Sufficient for Transforming Primary Rat Embryo Fibroblasts in Cooperation with the H-ras Oncogene. J. Virol. 72: 3060-3065 [Abstract] [Full Text]  
• Velu, T., Beguinot, L, Vass, W., Willingham, M., Merlino, G., Pastan, I, Lowy, D. (1987). Epidermal-growth-factor-dependent transformation by a human EGF receptor proto-oncogene. Science 238: 1408-1410 [Abstract]  
• Lufkin, T, Bancroft, C (1987). Identification by cell fusion of gene sequences that interact with positive trans-acting factors. Science 237: 283-286 [Abstract]  
• Lund, E, Kahan, B, Dahlberg, J. (1985). Differential control of U1 small nuclear RNA expression during mouse development. Science 229: 1271-1274 [Abstract]  
• Kasid, A, Lippman, M., Papageorge, A., Lowy, D., Gelmann, E. (1985). Transfection of v-rasH DNA into MCF-7 human breast cancer cells bypasses dependence on estrogen for tumorigenicity. Science 228: 725-728 [Abstract]  
• DeFeo-Jones, D, Tatchell, K, Robinson, L., Sigal, I., Vass, W., Lowy, D., Scolnick, E. (1985). Mammalian and yeast ras gene products: biological function in their heterologous systems. Science 228: 179-184 [Abstract]  
• Cooper, G. (1982). Cellular transforming genes. Science 217: 801-806 [Abstract]  
• Chang, E., Ellis, R., Scolnick, E., Lowy, D. (1980). Transformation by cloned Harvey murine sarcoma virus DNA: efficiency increased by long terminal repeat DNA. Science 210: 1249-1251 [Abstract]  
• Oskarsson, M, McClements, W., Blair, D., Maizel, J., Vande Woude, G. (1980). Properties of a normal mouse cell DNA sequence (sarc) homologous to the src sequence of Moloney sarcoma virus. Science 207: 1222-1224 [Abstract]  
• Liu, Y., Hong, Y., Androphy, E. J., Chen, J. J. (2000). Rb-independent Induction of Apoptosis by Bovine Papillomavirus Type 1 E7 in Response to Tumor Necrosis Factor alpha. J. Biol. Chem. 275: 30894-30900 [Abstract] [Full Text]