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J Virol. 1978 May; 26(2): 221-225

Transfection of Streptococcus pneumoniae with bacteriophage DNA.

C Ronda, R Lopez, A Tomasz and A Portoles

ABSTRACT

It was possible to transfect Streptococcus pneumoniae with DNA obtained from a newly isolated bacteriophage, diplophage-4 (Dp-4). Optimal frequency of transfection (0.9%) required the use of a nuclease-defective mutant; with wild-type bacteria, the transfection frequency was about 100-fold lower. Transfection requires physiological conditions that appear to be similar to the competent state needed for genetic transformation (A. Tomasz, J. Bacteriol. 91:1050--1061, 1966).

J Virol. 1978 May; 26(2): 221-225






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