This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nevins, J R Articles by Darnell, J E Search for Related Content PubMed PubMed Citation Articles by Nevins, J R Articles by Darnell, J E

 Previous Article  |  Next Article 

J Virol. 1978 March; 25(3): 811-823

Groups of adenovirus type 2 mRNA's derived from a large primary transcript: probable nuclear origin and possible common 3' ends.

ABSTRACT

Late in adenovirus type 2 infection, a number of mRNA's apparently arise by processing a large nuclear transcript that represents the right-hand 85% of the genome (summarized in Evans et al., Cell 12:733-739, 1977). Hybridization of labeled late mRNA to a series of DNA restriction fragments representing the right-hand 70% of the genome demonstrates at least 12 discrete mRNA's that appear to fall into five groups, each possibly containing a common 3' terminus. The processing necessary to generate these mRNA's apparently occurs in the nucleus. All the mRNA's appear to contain a sequence of approximately 100 nucleotides complementary to a fragment with coordinates 25.5-27.9. This fragment contains one of the regions found by Berget et al. (Proc. Natl. Sci. U.S.A. 74:3171-3175, 1977), Chow et al. (Cell 12:1-18, 1977), and Klessig (Cell 12:9-22, 1977) to the "spliced" onto the 5' end of late adenovirus type 2 mRNA's. Because the sequences to be spliced exist only once per large transcript, any of the mRNA-specific regions might only be preserved from a small fraction of the transcripts. Measurement of the transport efficiency of regions of the large nuclear transcript, if fact, shows that only 15 to 25% of any particular region is transported to the cytoplasm. The overall conclusion of these experiments is that the large late nuclear transcript can be processed in the nucleus to yield any one of many (approximately 12) mRNA's; the unused portions of the primary transcript then accumulate in the nucleus or are destroyed.

This article has been cited by other articles:

• Lupold, S. E., Kudrolli, T. A., Chowdhury, W. H., Wu, P., Rodriguez, R. (2007). A novel method for generating and screening peptides and libraries displayed on adenovirus fiber. Nucleic Acids Res 35: e138-e138 [Abstract] [Full Text]  
• Payet, V., Arnauld, C., Picault, J.-P., Jestin, A., Langlois, P. (1998). Transcriptional Organization of the Avian Adenovirus CELO. J. Virol. 72: 9278-9285 [Abstract] [Full Text]  
• Puvion-Dutilleul, F, Bachellerie, J., Visa, N, Puvion, E (1994). Rearrangements of intranuclear structures involved in RNA processing in response to adenovirus infection. J. Cell Sci. 107: 1457-1468 [Abstract]  
• Darnell, J. Jr (1978). Implications of RNA-RNA splicing in evolution of eukaryotic cells. Science 202: 1257-1260 [Abstract]