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J Virol. 1977 October; 24(1): 13-21
Copyright © 1977 American Society for Microbiology. All Rights Reserved.

Stimulation of Cellular Thymidine Kinases by Human Cytomegalovirus

John E. Estes and Eng-Shang Huang

1 Department of Medicine and The Cancer Research Center, School of Medicine, University of North Carolina, Chapel Hill, North Carolina 27514

ABSTRACT

Thymidine kinase (TK) activity in WI-38 and MRC-5 human fibroblasts was analyzed by discontinuous polyacrylamide gel electrophoresis (disc-PAGE) and discontinuous glycerol gradient electrophoresis (disc-GEP) after subculture or human cytomegalovirus (HCMV) infection. Two peaks of TK activity with different relative fraction-of-migration (Rf) values were resolved by disc-PAGE or disc-GEP in extracts from log-phase and infected cells. Growing WI-38 cells expressed a slowly migrating (Rf = 0.14 PAGE, Rf = 0.4 GEP) peak of TK activity, which was partially inhibited by 1.0 mM dCTP, but which retained little activity at pH 4.5. Growing MRC cells also displayed a slowly migrating peak (Rf = 0.10 PAGE) with similar properties. Both cell types expressed a faster-migrating TK activity (Rf = 0.45 PAGE, Rf = 0.7 GEP) in the growing and resting state that was strongly inhibited by 1 mM dCTP but retained 50% activity at pH 4.5. When either cell type was infected with HCMV, there was a rapid and high-level stimulation of the slowly migrating form of TK and a slight stimulation of the faster-migrating form. Two strains of HCMV (AD169 and Town) failed to produce an electrophoretically distinct virus TK in either cell type after infection. TK enzymes were partially purified by disc-GEP from extracts of log-phase WI-38 or AD169-infected WI-38 cells. Characterization of these enzymes with respect to phosphate donor specificity, pH optima, thermostability, and salt inhibition showed the HCMV-stimulated TKs to be of cellular origin.

J Virol. 1977 October; 24(1): 13-21
Copyright © 1977 American Society for Microbiology. All Rights Reserved.



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