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J Virol. 1977 August; 23(2): 240-249
Copyright © 1977 American Society for Microbiology. All Rights Reserved.

Species Identification and Genome Mapping of Cytoplasmic Adenovirus Type 2 RNAs Synthesized Late in Infection

Michael McGrogan and Heschel J. Raskas

1 Department of Pathology and Department of Microbiology and Immunology, Division of Biology and Biomedical Sciences, Washington University School of Medicine, St. Louis, Missouri 63110

ABSTRACT

Adenovirus type 2 cytoplasmic RNAs synthesized late in productive infection were resolved by electrophoresis on formamide gels. Regions of the adenovirus 2 genome specifying RNAs of distinct size were determined by hybridization to specific DNA fragments generated by cleavage with endo R·EcoRI and endo R·SmaI. From these studies 13 distinct viral RNA species were identified. A 26S to 28S size class and a 21S to 23S size class were each found to consist of four distinct RNA species. Three RNA species were identified in a 16S to 18S size class, and a fourth size class, 11S to 13S, was resolved into two components. The SmaI-D region (0.38 to 0.51 on the unit genome) and the EcoRI-F, D region (0.70 to 0.83) of the genome were found to code for multiple transcripts. Three RNAs (28S, 22S, and 18S) are specified by SmaI-D, and four components, 28S, 22S, 18S, and 16S, are encoded by EcoRI-F,D. The RNA represented by each set of multiple transcripts exceeds the coding capacity of the respective region, and the species within each set of RNAs appear to contain common sequences. The relationship between the cytoplasmic RNA species synthesized at late times and early cytoplasmic RNAs was determined by hybridization-inhibition experiments. The multiple transcripts encoded by the EcoRI-D fragment were found to contain sequences that are present in early cytoplasmic RNA. These studies enabled preparation of a map which accounts for transcription of approximately 67% of the r strand of the adenovirus 2 genome.

J Virol. 1977 August; 23(2): 240-249
Copyright © 1977 American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jimenez-Garcia, L., Green, S., Mathews, M., Spector, D. (1993). Organization of the double-stranded RNA-activated protein kinase DAI and virus-associated VA RNAI in adenovirus-2-infected HeLa cells. J. Cell Sci. 106: 11-22 [Abstract]  
  • Spector, D. J., Halbert, D. N., Crossland, L. D., Raskas, H. J. (1980). Expression of Genes from the Transforming Region of Adenovirus. Cold Spring Harb Symp Quant Biol 44: 437-445 [Abstract]  


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