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J Virol. 1976 November; 20(2): 527-531

Anatomy of herpes simplex virus DNA. VI. Defective DNA originates from the S component.

N Frenke, H Locker, W Batterson, G S Hayward and B Roizman

ABSTRACT

We previously reported that serial propagation of the Justin strain of herpes simplex virus 1 [HSV-1 (Justin)] results in the generation of defective DNA molecules consisting of tandem repetitions of sequences of limited complexity. In the present study, HSV-1 DNA was cleaved with the restriction endonucleases BglII and EcoRI. The fragments were electrophoretically separated on agarose gels, transferred to nitrocellulose strips, and then hybridized with 32P-labeled HSV-1 (Justin) defective DNA. The data allow us to conclude that DNA sequences contained in the repeat unit of defective DNA originate from the S segment of the wild-type viral DNA molecule.

This article has been cited by other articles:

• Roizman, B, Jenkins, F. (1985). Genetic engineering of novel genomes of large DNA viruses. Science 229: 1208-1214 [Abstract]  
• Enquist, L., Madden, M., Schiop-Stanley, P, Vande Woude, G. (1979). Cloning of herpes simplex type 1 DNA fragments in a bacteriophage lambda vector. Science 203: 541-544 [Abstract]