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J Virol. 1968 September; 2(9): 869-877
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Reovirus-induced Ribonucleic Acid Polymerase

The Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104

ABSTRACT

A virus-induced ribonucleic acid (RNA) polymerase activity was found in L cells infected with type 3 reovirus. Most of the enzyme is associated with the "large particle" fraction of the infected cells. The enzyme first appeared at 3 to 5 hr after infection and increased in amount until 7 to 9 hr. All four ribonucleoside triphosphates are incorporated in vitro into an acid-insoluble form by the enzyme. The major part of the product formed in vitro is a double-stranded RNA indistinguishable from viral RNA by electrophoresis on polyacrylamide gel. Approximately 40% of the product is a single-stranded RNA of relatively small molecular weight. More than 95% of the nucleotides incorporated into double-stranded RNA by the enzyme are bound in internal 3'-5'-phosphodiester linkages extending back from both 3'- and 5'-termini of the RNA strands.

¹ Postdoctoral Fellow of the Damon Runyon Memorial Fund for Cancer Research.