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In Vitro Morphology and Maturation of Lymphocystis Virus ¹

Department of Biology, Lehigh University, Bethlehem, Pennsylvania 18015

ABSTRACT

The temporal sequence of development of lymphocystis disease virus (LDV) was studied by electron microscopy of thin sections of infected tissue-culture monolayers. Neither the typical cytoplasmic inclusion nor virus was detected at 4 days postinfection (PI). Inclusions, but no viruses, were detected at 8 days PI. Inclusions and associated virions were detected at 15 days PI, and by 28 days PI the undisrupted cells were filled with the typical virions. No release mechanism was detected, and severe clumping of particles was noted. Negatively stained preparations revealed particles 200 nm in diameter with no capsomere structure and apparent spikes associated with the particle. The relationship of LDV to the well-defined deoxyribonucleic acid virus groups is discussed.

² Present address: Department of Biology, Fairleigh Dickinson University, Madison, N.J. 07940.

¹ Results presented comprise a portion of a dissertation submitted by F. H. Midlige, Jr., to Lehigh University in partial fulfillment of the requirements for the Ph.D. degree.