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J Virol. 1968 August; 2(8): 787-792
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Vaccinia Virus Replication and Cytopathic Effect in Cultures of Phytohemagglutinin-treated Human Peripheral Blood Leukocytes

George Miller and John F. Enders

The Research Division of Infectious Diseases, The Children's Hospital Medical Center, The Department of Medicine and The Department of Bacteriology and Immunology, Harvard Medical School, Boston, Massachusetts 02115

ABSTRACT

Titers of vaccinia virus consistently increased in cultures of washed phytohemagglutinin-treated, peripheral blood leukocytes of a vaccinated adult. Concomitantly, a gradual rise occurred in the numbers of infected leukocytes, as determined by the infective center assay. Increase in viral titer was accompanied by cell injury, decline in cell numbers, and decreased acid production. Leukocytes not pretreated with phytohemagglutinin appeared to form infective centers after exposure to the vaccinia agent, but they did not replicate infectious virus. For viral replication, the continuous presence of phytohemagglutinin was required.

J Virol. 1968 August; 2(8): 787-792
Copyright © 1968 American Society for Microbiology. All Rights Reserved.



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Copyright © 1968 by the American Society for Microbiology. All rights reserved.