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J Virol. 1968 July; 2(7): 657-662
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Transducing Bacteriophage for Bacillus cereus

Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01002

ABSTRACT

A phage, designated CP-51, that carries out generalized transduction in Bacillus cereus 569 was isolated from soil. All auxotrophic mutants tested, those requiring tryptophan, histidine, leucine, isoleucine, methionine, or phenylalanine, were transduced to prototrophy. The phage was extremely unstable when stored at 2 to 4 C, but stability was enhanced by storage at higher temperatures. The optimal temperature of those tested for maintenance of plaque-forming units was 15 C.

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