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Mechanism of Resistance to Enteroviruses of Some Primate Cells in Tissue Culture

N. F. Gamaleya Institute for Epidemiology and Microbiology, Academy of Medical Sciences, Moscow State University, Moscow, USSR
Institute of Poliomyelitis and Viral Encephalitides, Academy of Medical Sciences, Moscow State University, Moscow, USSR
Department of Virology, Moscow State University, Moscow, USSR

ABSTRACT

Two cell lines, M10-45-2 and L-41, were studied, each of which possessed specific resistance either to poliovirus or to coxsackievirus. Infection of M10-45-2 cells with poliovirus ribonucleic acid (RNA) and L-41 cells with infectious coxsackievirus RNA was accompanied by production of complete viruses in each of the resistant cell lines. During incubation of the cells with the virus to which they were resistant, the amount of infectious virus did not decrease. Treatment with glycine-HCl buffer solution (pH 2.5) of resistant M10-45-2 cells after incubation with poliovirus at 0 C did not result in recovery of infectious virus, although such release did take place after treatment of sensitive M10 cells. Infection of resistant cells with virus containing poliovirus RNA and coxsackievirus proteins resulted in production of poliovirus in M10-45-2 cells but not in L-41 cells. The resistant cells are apparently unable to adsorb the virus to which they are resistant.