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J Virol. 1968 June; 2(6): 547-552
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Replicative Intermediate of an Arbovirus

Robert M. Friedman

Laboratory of Pathology, National Cancer Institute, Bethesda, Maryland 20014

ABSTRACT

One hour after infection of chick fibroblasts with Semliki Forest virus (SFV), a viral ribonucleic acid (RNA) structure is present which has many of the properties described for the replicative intermediate of several RNA bacteriophages. These properties include a polydisperse nature on sucrose density gradient analysis, ribonuclease resistance, a variation in sedimentation pattern associated with changes in salt concentration, and recovery of infectious viral RNA upon denaturation. Most of the replicative intermediate present in SFV infection appears to be membrane-associated.

J Virol. 1968 June; 2(6): 547-552
Copyright © 1968 American Society for Microbiology. All Rights Reserved.





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Copyright © 1968 by the American Society for Microbiology. All rights reserved.