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Electron Microscopy of Herpes Simplex Virus

II. Sequence of Development

College of Physicians and Surgeons, Columbia University, New York, New York 10032

ABSTRACT

Examination of infected cells at sequential intervals after infection revealed that the first viral forms to appear were capsids enclosing cores of low density. Not until the 6th hr were dense cores encountered, and at approximately the same time enveloped virus was seen. Envelopment occurred most frequently in close proximity to the nuclear surface, although the process was also encountered within the nuclear matrix and in the cytoplasm. There was often extensive proliferation of the nuclear membrane. Envelopment of the virus by budding from the cell surface was not observed. It was concluded that enveloped virus consitutes the infectious particle and that the unenveloped capsid is unstable outside the cell. Nevertheless, it is likely that capsids enclosing infectious nucleic acid can pass directly from one cell to another after fusion has taken place.

¹ Postdoctoral Fellow supported by grants from the Rockefeller Foundation, a special Lederle International Fellowship, and The Jane Coffin Childs Memorial Fund for Medical Research. Present address: The Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.

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