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Rescue of Temperature-sensitive Poliovirus ¹

Department of Microbiology, The Pennsylvania State University, University Park, Pennsylvania 16802

ABSTRACT

A temperature-sensitive strain of type 1 poliovirus, LSc, was functionally rescued when infected cells were incubated at 40 C in the presence of Mahoney, a temperature-resistant strain of type 1 poliovirus. The rescue value was 9% of the mutant yield obtained under permissive conditions. Rescued virus underwent replication, because the progeny of ³²P-labeled LSc were not radiosensitive. Serum inactivation studies with Mahoney specific antiserum indicated that a small amount of phenotypic mixing occurred among the rescued particles. The temperature-sensitive event occurred between 2 and 4 hr postinfection in the developmental cycle of LSc. Neither viral polymerase activity nor virus-induced ribonucleic acid could be demonstrated in infected cells between 2 and 4 hr after infection at 40 C with the temperature-sensitive mutant.

² Present address: Virology Research, The Upjohn Co., Kalamazoo, Mich. 49001.

¹ Paper No. 3350 in the Journal Series of the Pennsylvania Agricultural Experiment Station, University Park, authorized for publication on 8 January 1968.