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J Virol. 1968 March; 2(3): 248-255
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Isolation and Properties of Newcastle Disease Virus Nucleocapsid

D. W. Kingsbury and R. W. Darlington

Laboratory of Virology, St. Jude Children's Research Hospital, and The University of Tennessee Medical Units, Memphis, Tennessee 38101

ABSTRACT

Deoxycholate (DOC) disrupted virions of Newcastle disease virus (NDV), releasing viral nucleocapsids. The nucleocapsids sedimented at about 200S in sucrose gradients and measured from 1.3 to 1.4 µ long by electron microscopy. NDV nucleo-capsids were resistant to pancreatic ribonuclease. These nucleocapsids contained all the 50S ribonucleic acid (RNA) in NDV virions, while virus-associated RNA sedimenting at less than 50S was external to the virions.

J Virol. 1968 March; 2(3): 248-255
Copyright © 1968 American Society for Microbiology. All Rights Reserved.





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J. Bacteriol. Mol. Cell. Biol. Microbiol. Mol. Biol. Rev.
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Copyright © 1968 by the American Society for Microbiology. All rights reserved.