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Enhancement of St. Louis Arbovirus Plaque Formation by Neutral Red

Division of Laboratories, Pennsylvania Department of Health, and Temple University School of Medicine, Philadelphia, Pennsylvania 19130

ABSTRACT

Experiments with St. Louis encephalitis (SLE) virus have shown that neutral red enhances the plaque size in duck embryo cell cultures. This may represent a new method for genetic studies of SLE virus population. In a mosquito pool specimen NR⁺ and NR^– particles were demonstrated. By intracerebral passage in mice, there is a selection of NR⁺ particles. Similar effects were not shown for eastern, western, and California encephalitis viruses. Plaques formed by the latter virus, however, were significantly reduced in number by neutral red.

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