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J Virol. 1968 December; 2(12): 1363-1367
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Induction of Poxvirus Ribonucleic Acid Polymerases

^a Department of Microbiology, University of California Medical Center, San Francisco, California 94122

ABSTRACT

Two distinct ribonucleic acid polymerase activities were induced in HeLa cells by poxvirus infection. These activities differ both in their properties and the time of their appearance after infection. One catalyzes the dAT (copolymer of deoxyadenylate and deoxythymidylate)-primed conversion of adenosine triphosphate and uridine triphosphate into an acid-insoluble product. This enzyme is detectable only if deoxyribonucleic acid synthesis has been blocked. In contrast, the accumulation of progeny genomes is a necessary condition for induction of the second enzyme. The latter activity, which is unmasked by detergent treatment, is found exclusively in maturing virus particles. The possibility that both enzymes are involved in transcribing the viral genome is discussed.

¹ U.S. Public Health Service postdoctoral fellow (1-F2-CA-37,249-01).

² On leave from the Roche Institute for Molecular Biology.

³ U.S. Public Health Service predoctoral trainee.

⁴ Recipient U.S. Public Health Service Career Development Award (1-K4-AI-11,820-01).