This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pizer, L. I. Articles by Pylkas, L. Search for Related Content PubMed PubMed Citation Articles by Pizer, L. I. Articles by Pylkas, L.

 Previous Article  |  Next Article 

J Virol. 1968 November; 2(11): 1339-1345
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Effect of Prophage W on the Propagation of Bacteriophages T2 and T4

¹ Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104

ABSTRACT

Studies have been undertaken to determine whether the temperate phage present in Escherichia coli strain W is responsible for the inability of this strain to act as a host for T2 and T4. E. coli WS, cured of phage , was sensitive to T2 and T4. Lysogenation of E. coli C and WS with phage resulted in loss of ability to plate T2 and T4. However, E. coli K-12 lysogens still served as hosts for the T -even phage. Two of three WS lysogens studied resembled strain W at the biochemical level. They converted about 30% of infecting T2 deoxyribonucleic acid (DNA) to acid-soluble fragments and limited macromolecular synthesis to a few minutes after infection. The third lysogen did not degrade phage DNA, and nucleic acid and protein synthesis continued for some time, although no phage production occurred. It is concluded that phage plays a role in the restriction of virulent phage but that it is not the only factor involved. Since acid solubilization was not observed in all cases of phage -mediated restriction of T -even phage, a hypothesis for the restriction has been proposed which is based on an alteration in the cell envelope after lysogenation with phage .

This article has been cited by other articles:

• Karlsson, J. L., Cardoso-Palacios, C., Nilsson, A. S., Haggard-Ljungquist, E. (2006). Evolution of Immunity and Host Chromosome Integration Site of P2-Like Coliphages. J. Bacteriol. 188: 3923-3935 [Abstract] [Full Text]  
• Liu, T., Haggård-Ljungquist, E. (1999). The Transcriptional Switch of Bacteriophage WPhi , a P2-Related but Heteroimmune Coliphage. J. Virol. 73: 9816-9826 [Abstract] [Full Text]