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J Virol. 1968 November; 2(11): 1326-1338
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Cytoplasmic Structures Associated with an Arbovirus Infection: Loci of Viral Ribonucleic Acid Synthesis

¹ Pathologic Anatomy Branch and Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014

ABSTRACT

Unique cytoplasmic structures, herein designated as type I cytopathic vacuoles (CPV-I), are found in chick embryo cells early in the logarithmic phase of Semliki Forest virus replication. High resolution autoradiography demonstrated that the CPV-I are loci of ³H-uridine incorporation. This evidence correlates well with previous biochemical data and electron microscopy of the subcellular fractions active in Semliki Forest virus ribonucleic acid synthesis. Origin of the CPV-I within host cell cytoplasm is confirmed by the distribution of electron-dense tracer particles and sequential ultrastructural observations.

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