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Host Cell Participation in Small Virus Replication I. Replication of M-13 in a Strain of Escherichia coli with a Temperature-sensitive Lesion in Deoxyribonucleic Acid Synthesis

Department of Bacteriology, University of California, Davis, California 95616
Department of Microbiology, Tulane University School of Medicine, New Orleans, Louisiana 70112
Department of Bacteriology, University of California, Davis, California 95616

ABSTRACT

The replication of M-13 in a strain of Escherichia coli with a thermosensitive lesion in deoxyribonucleic acid synthesis was studied. M-13 failed to replicate at the restrictive temperature, even when the parental replicative form was allowed to form at the permissive temperature. When cells which were actively producing phage at the permissive temperature were shifted to the restrictive temperature, phage production continued. The incorporation of radioactive label into phage particles at 42 C indicated that continued single-strand synthesis was unaffected by the lesion in the host cell.

¹ Present address: Department of Biochemistry and Biophysics, University of California, Davis 95616.