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J Virol. 1968 November; 2(11): 1296-1307
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Replication of Coliphage M-13 II. Intracellular Deoxyribonucleic Acid Forms Associated with M-13 Infection of Mitomycin C-treated Cells1

Lyle R. Brown2 and C. E. Dowell

Department of Microbiology, Tulane University School of Medicine, New Orleans, Louisiana 70112
Department of Bacteriology, University of California, Davis, California 95616

ABSTRACT

Intracellular deoxyribonucleic acid (DNA) forms associated with bacteriophage M-13 infection have been isolated and characterized. Escherichia coli HF4704 (F+, hcr–, thy–) cells were treated with mitomycin C to inhibit host-cell DNA synthesis and were then infected with phage M-13. This treatment permitted radioactive labeling of phage-specific DNA forms with 3H-thymine. These labeled DNA components were characterized by sucrose density sedimentation and equilibrium density gradient centrifugation in neutral and ethidium bromide CsCl gradient. Two double-stranded circular forms were found with properties analogous to the replicative form I and replicative form II of {varphi}X174. A third component, identified as single-stranded DNA, was isolated in some samples removed 45 min after phage synthesis was initiated.

FOOTNOTES

2 Present Address: Department of Bacteriology, University of California, Davis 95616.

1 Taken from a dissertation submitted by L. R. B. to Tulane University in partial fulfillment of the requirement for the Ph.D. degree, May 1968.

J Virol. 1968 November; 2(11): 1296-1307
Copyright © 1968 American Society for Microbiology. All Rights Reserved.





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