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J Virol. 1968 November; 2(11): 1290-1295
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Replication of Coliphage M-13 I. Effects on Host Cells After Synchronized Infection¹

Department of Microbiology, Tulane University School of Medicine, New Orleans, Louisiana 70112
Department of Bacteriology, University of California, Davis, California 95616

ABSTRACT

Techniques have been described for synchronization of bacteriophage M-13 infection of host cells. The latent period in infected cells was 10 min, and no appreciable number of intracellular phage was observed. Phage production proceeded in three phases after release of the starvation block: an initial rapid exponential rate of progeny phage release without cell lysis, a period of rate transition accompanying the resumption of host cell division, and a second, slower exponential rate of phage production which paralleled the rate of host cell division. The size of infected cells was not affected by infection, but the generation time was increased by 25%. Starved infected cells exhibited a much longer lag in attaining an exponential rate of growth upon the addition of nutrients than did an uninfected control culture.

² Present Address: Department of Bacteriology, University of California, Davis 95616.

¹ Taken from a dissertation submitted by L. R. B. to Tulane University in partial fulfillment of the requirement for the Ph.D. degree, May 1968.

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