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J Virol. 1968 October; 2(10): 1185-1190
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Physical and Biological Properties of Phage 29 Deoxyribonucleic Acid

Department of Microbiology and School of Dentistry, University of Minnesota, Minneapolis, Minnesota 55455

ABSTRACT

Deoxyribonucleic acid (DNA) molecules having a mean length of 5.8 µm were released from purified Bacillus subtilis bacteriophage 29 with 2 M sodium perchlorate. Small 0.1 to 0.2-µm molecules were also detected in these DNA preparations. Since intact single chains annealed to form linear duplex molecules, phage 29 DNA was found to be nonpermuted. The molecular weights of single chains of 29 DNA were approximately half that of native DNA, as determined by analytical band sedimentation in CsCl, indicating that 29 DNA is composed of two continuous polynucleotide chains. The molecular weight values of native and annealed 29 DNA from sedimentation agreed with the molecular weight values obtained from electron microscopy. The infectivity of 29 DNA was reduced to a low level by alkaline denaturation and was partially restored by annealing.