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J Virol. 1968 October; 2(10): 1064-1075
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Virus-Receptor Interaction in an Adenovirus System

^a Department of Microbiology, The Wallenberg Laboratory, Uppsala University, Uppsala, Sweden

ABSTRACT

HeLa or KB cells each contain around 10⁴ receptor sites for adenovirus type 2. These are inactivated by treatment of live cells with subtilisin. The receptor activity of the enzyme-treated cells is regained after 4 to 8 hr of incubation in complete medium. A technique that utilizes the difference in buoyant density between free virus and virus-receptor complex was developed to demonstrate receptor activity. Cellular fractionation revealed that receptors were confined mainly to the plasma membrane fraction and that negligible receptor activity could be demonstrated in enzyme-treated cells. Subtilisin probably did not penetrate the cell membrane; thus, the receptors are limited to the cell surface. Purified fiber of the virion completely prevents attachment of adenovirus types 2 and 5 to receptor sites at a ratio of 10⁵ protein molecules per cell. Adsorption studies indicate that 10⁵ to 10⁶ receptor sites are available for the structural protein. The fiber does not affect attachment of poliovirus type 1.

¹ Recipient of special National Institutes of Health Research Fellowship Award 5F3-AI-6864.

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