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Characterization of the Tumorlike (T) Antigen Induced by Type 12 Adenovirus ¹

I. Purification of the Antigen from Infected KB Cells and a Hamster Tumor Cell Line

Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104

ABSTRACT

The T antigen induced by type 12 adenovirus was purified from KB cells infected in the presence of 10^–6M 5-fluoro-2-deoxyuridine to inhibit synthesis of viral capsid antigens. The antigen was purified approximately 200-fold, and the purified product contained only negligible amounts of host-cell contaminants, as judged by the residual radioactivity from ¹⁴C-labeled uninfected cells which had been added to infected cells at the initiation of the purification. Immunoelectrophoresis indicated that the purified T-antigen preparation contained a single antigenic species. The T antigen from a hamster cell line (HT-1) derived from a type 12 adenovirus-induced tumor was purified by the same procedure. The T antigens from the two different sources were shown to be immunologically similar by use of a rabbit antiserum prepared against the purified T antigen from infected KB cells and sera from hamsters bearing tumors induced by type 12 adenovirus.

² Present address: Section of Genetics, The Weizmann Institute of Science, Rehovoth, Israel.

¹ Presented in part at the 67th annual meeting of the American Society for Microbiology, New York, N.Y. 1967.