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J Virol. 1968 January; 2(1): 69-77
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Selection of Morphologically Normal Cell Lines from Polyoma-transformed BHK21/13 Hamster Fibroblasts

International Laboratory of Genetics and Biophysics, Naples, Italy

ABSTRACT

A selective method was devised for the isolation of "revertants" from polyoma-transformed sublines derived from BHK21/13 Syrian hamster fibroblasts. A hybrid, polyploid subline was obtained by growing together, in mixed culture in the presence of aminopterin, two variant BHK21/13 sublines lacking either inosinic acid pyrophosphorylase or thymidine kinase. Whereas these variant sublines were resistant to 6-thioguanine or to 5-bromodeoxyuridine, the hybrid had regained sensitivity to both analogues. By plating a polyoma-transformed subline derived from this hybrid in the presence of 6-thioguanine, resistant clones were obtained with a frequency of about 10^–4. All of these surviving clones had a reduced chromosome complement and some of them had regained a normal phenotype.

¹ Present address: The Massachusetts General Hospital Boston, Mass. 02114.

This article has been cited by other articles:

• Dulbecco, R. (1969). Cell Transformation by Viruses. Science 166: 962-968