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J Virol. 1968 January; 2(1): 48-55
Copyright © 1968 American Society for Microbiology. All Rights Reserved.

Herpesvirus Envelopment 1

Robert W. Darlington and L. Howard Moss III2

Laboratory of Virology, St. Jude Children's Research Hospital, and Department of Microbiology, University of Tennessee Medical Units, Memphis, Tennessee 38101

ABSTRACT

The growth and envelopment processes of three representative herpesviruses, equine abortion, pseudorabies, and herpes simplex, were examined in baby hamster kidney (BHK 21/13) cells by bioassay (plaque-forming units) and electron microscopy. The envelopment process was identical for all three viruses. After assembly in the nucleus, the nucleocapsid acquired an envelope by budding from the inner nuclear membrane. This membrane was reduplicated as the enveloped particle was released so that the budding process did not result in disruption of the continuity of the nuclear membrane. That portion of the nuclear membrane which comprised the viral envelope was appreciably thicker than the remainder of the membrane and exhibited numerous projections on its surface. Once enveloped, the viral particles were seen in vesicles and vacuoles in the cell cytoplasm. These appeared to open at the cytoplasmic membrane, releasing the virus from the cell. There was no detectable difference in the size or appearance of enveloped particles in intra- or extracellular locations.

FOOTNOTES

2 The results presented here comprise a portion of a dissertation submitted to the University of Tennessee Medical Units in partial fulfillment of requirements for the degree of Doctor of Philosophy.

1 Presented in part at the annual meetings of the American Society for Microbiology, New York, N. Y., May 1967, and the Tissue Culture Association, Philadelphia, Pa., June 1967.

J Virol. 1968 January; 2(1): 48-55
Copyright © 1968 American Society for Microbiology. All Rights Reserved.



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Copyright © 1968 by the American Society for Microbiology. All rights reserved.