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Characterization of the Tumorlike (T) Antigen Induced by Type 12 Adenovirus ¹

II. Physical and Chemical Properties

Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104

ABSTRACT

T antigen, purified from KB cells infected with type 12 adenovirus, was characterized by chemical and physical methods. The immunologically active molecule had an ultraviolet-absorption spectrum characteristic of a protein, and was partially inactivated (i.e., approximately 85%) by trypsin (final concentration of 150 µg/ml). Centrifugation analyses indicated that the purified antigen was a single molecular species with an average sedimentation coefficient of 2.40S (measured by zonal centrifugation in sucrose gradients) and an S_20,w of 2.58 determined by analytical centrifugation. The antigen was inactivated at temperatures above 40 C, it was stable between pH 5.7 and 8.8, and it had an isoelectric point of approximately 5.0, as determined by electrophoresis on cellulose polyacetate membranes.

² Present address: Section of Genetics, The Weizmann Institute of Science, Rehovoth, Israel.

¹ Presented in part at the 67th Annual Meeting of the American Society for Microbiology, New York, N.Y., 1967.