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J Virol. 1976 September; 19(3): 899-902
Copyright © 1976 American Society for Microbiology. All Rights Reserved.

Biochemical and Biophysical Properties of Hyphomicrobium Bacteriophage Hy30

^a Department of Microbiology, West Virginia University Medical Center, Morgantown, West Virginia 26506

ABSTRACT

Hyphomicrobium bacteriophage Hy30 and its nucleic acid were studied to determine some of their biochemical and biophysical properties. The molecular weight of the phage is 55.4 x 10⁶, and its buoyant density is 1.508 g/ml. The nucleic acid of Hy30 is linear, double-stranded DNA with a molecular weight of 29.7 x 10⁶. The guanine-plus-cytosine content of the DNA was 62% as determined from its melting temperature and buoyant density.

¹ Present address: Department of Medicine, University of Chicago, Chicago, IL 60637.