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J Virol. 1976 May; 18(2): 799-803
Copyright © 1976 American Society for Microbiology. All Rights Reserved.

Spontaneous Release of Endogenous Ecotropic Type C Virus from Rat Embryo Cultures

Suraiya Rasheed*, Joan Bruszewski, Robert W. Rongey, Pradip Roy-Burman, Howard P. Charman1 and Murray B. Gardner

* Department of Pathology, University of Southern California School of Medicine, Los Angeles, California 90033
Department of Biochemistry, University of Southern California School of Medicine, Los Angeles, California 90033

ABSTRACT

Type C viruses were isolated from embryo cultures of two different rat strains, Sprague-Dawley and Fischer. Both viruses (termed rat leukemia virus, RaLV) were released spontaneously from rat embryo cells, have a density of 1.14 to 1.15 g/cm3 based on equilibrium sedimentation in sucrose gradients, contain 60-70S RNA, RNA-directed DNA polymerase, and rat type C virus-specific 30,000 molecular-weight-protein determinants. Molecular hybridization studies using the Sprague-Dawley RaLV 60-70S RNA show that the virus-specific nucleotide sequences are present in the DNA of rat embryos. Both Sprague-Dawley and Fischer RaLV can rescue the murine sarcoma virus genome from Kirsten murine sarcoma virus-transformed nonproducer cells and are neutralized by antisera to the RPL strain of RaLV. In contrast to previous RaLV's, these viruses propagate in their own cells of origin as well as in cells of heterologous rat strains.

FOOTNOTES

1 Present address: Flow Laboratories, Inc., Rockville, Md. 20852.

J Virol. 1976 May; 18(2): 799-803
Copyright © 1976 American Society for Microbiology. All Rights Reserved.



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