This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Summers, W C Articles by Klein, G Search for Related Content PubMed PubMed Citation Articles by Summers, W C Articles by Klein, G

Inhibition of Epstein-Barr virus DNA synthesis and late gene expression by phosphonoacetic acid.

ABSTRACT

Growth of lymphoblastoid cells (B95-8, Raji) is not inhibited by the presence of 0.4 mM phosphonoacetic acid. The synthesis of Epstein-Barr virus (EBV) in the producer line B95-8 is completely inhibited, as shown by the total inhibition of viral capsid antigen synthesis. Early viral antigens are made normally in the presence of phosphonoacetic acid, but EBV DNA synthesis is blocked in cells entering the productive cycle. Nonproducer cells in the population replicate the resident EBV DNA by a mechanism that is resistant to phosphonoacetic acid. These results are consistant with the hypotheses that EBV DNA is replicated by two mechanisms, one in the noninduced cell and a different mechanism in the producer cell, and that prior replication of EBV DNA, probably by the second mode, is a prerequisite for late gene expression.

This article has been cited by other articles:

• Amon, W., White, R. E., Farrell, P. J. (2006). Epstein-Barr virus origin of lytic replication mediates association of replicating episomes with promyelocytic leukaemia protein nuclear bodies and replication compartments.. J. Gen. Virol. 87: 1133-1137 [Abstract] [Full Text]  
• El-Guindy, A. S., Miller, G. (2004). Phosphorylation of Epstein-Barr Virus ZEBRA Protein at Its Casein Kinase 2 Sites Mediates Its Ability To Repress Activation of a Viral Lytic Cycle Late Gene by Rta. J. Virol. 78: 7634-7644 [Abstract] [Full Text]  
• Tang, S., Yamanegi, K., Zheng, Z.-M. (2004). Requirement of a 12-Base-Pair TATT-Containing Sequence and Viral Lytic DNA Replication in Activation of the Kaposi's Sarcoma-Associated Herpesvirus K8.1 Late Promoter. J. Virol. 78: 2609-2614 [Abstract] [Full Text]  
• Gradoville, L., Gerlach, J., Grogan, E., Shedd, D., Nikiforow, S., Metroka, C., Miller, G. (2000). Kaposi's Sarcoma-Associated Herpesvirus Open Reading Frame 50/Rta Protein Activates the Entire Viral Lytic Cycle in the HH-B2 Primary Effusion Lymphoma Cell Line. J. Virol. 74: 6207-6212 [Abstract] [Full Text]  
• Zacny, V. L., Gershburg, E., Davis, M. G., Biron, K. K., Pagano, J. S. (1999). Inhibition of Epstein-Barr Virus Replication by a Benzimidazole L-Riboside: Novel Antiviral Mechanism of 5,6-Dichloro-2-(Isopropylamino)-1-beta -L-Ribofuranosyl-1H-Benzimidazole. J. Virol. 73: 7271-7277 [Abstract] [Full Text]  
• Babcock, G. J., Decker, L. L., Freeman, R. B., Thorley-Lawson, D. A. (1999). Epstein-Barr Virus–infected Resting Memory B Cells, not Proliferating Lymphoblasts, Accumulate in the Peripheral Blood of Immunosuppressed Patients. J. Exp. Med. 190: 567-576 [Abstract] [Full Text]  
• Sun, R., Lin, S.-F., Staskus, K., Gradoville, L., Grogan, E., Haase, A., Miller, G. (1999). Kinetics of Kaposi's Sarcoma-Associated Herpesvirus Gene Expression. J. Virol. 73: 2232-2242 [Abstract] [Full Text]  
• Serio, T. R., Cahill, N., Prout, M. E., Miller, G. (1998). A Functionally Distinct TATA Box Required for Late Progression through the Epstein-Barr Virus Life Cycle. J. Virol. 72: 8338-8343 [Abstract] [Full Text]