This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Davis, D B Articles by Kingsbury, D T Search for Related Content PubMed PubMed Citation Articles by Davis, D B Articles by Kingsbury, D T

 Previous Article  |  Next Article 

J Virol. 1976 March; 17(3): 788-793

Quantitation of the viral DNA present in cells transformed by UV-irradiated herpes simplex virus.

ABSTRACT

Two cell lines biochemically transformed by UV-irradiated herpes simplex virus (HSV) each contain virus DNA. A comparison of the kinetics of reassociation of 3H-labeled HSV DNA in the presence and absence of either clone 139 (HSV-1 transformed) or clone 207 (HSV-2 transformed) DNA showed that the presence of transformed cell DNA increased the rate of reassociation of approximately 10% of the viral genome while having no effect on the remaining 90%. The Cot1/2 of this reaction was approximately 1,000 in each cell type, as compared to approximately 3,000 for the cellular unique sequences. These results suggest the presence of four to six copies of a 10% fragment of the virus DNA per cell. The DNA from a hamster fibroblast cell line morphologically transformed by UV-irradiated HSV-2 (333-8-9) did not affect the rate of reassociation of HSV-2 DNA, indicating that these cells had less than 3% of a viral genome present.

This article has been cited by other articles:

• Jenson, H. B., Robert, M. F. (1987). Congenital Cytomegalovirus Infection With Osteolytic Lesions: Use of DNA Hybridization in Diagnosis. CLIN PEDIATR 26: 448-452 [Abstract]