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J Virol. 1976 February; 17(2): 668-671

Immunocytochemical localization of simian virus 40 T antigen with peroxidase-labeled antibody fragments.

K Tabuchi, J M Lehman and W M Kirsch

ABSTRACT

T antigen was localized in simian virus 40 lytically infected and transformed cells by Fab' antibody conjugates at the ultrastructural level. This virus-specific protein appeared in the cytoplasm of permissive cells as early as 3 h after infection. At later time intervals, the T antigen was localized in both the cytoplasm and nucleus and finally (24 h) in the nucleus. These results suggest a synthesis of T antigen on cytoplasmic ribosomes, with subsequent transfer to the nucleus.

J Virol. 1976 February; 17(2): 668-671






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