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Comparison of RNA Polymerase Associated With Newcastle Disease Virus and a Temperature-Sensitive Mutant of Newcastle Disease Virus Isolated from Persistently Infected L Cells

^a Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, Louisiana 70112

ABSTRACT

An in vitro comparison was made of the RNA polymerase activity associated with Newcastle disease virus (NDVo) and three clones of the temperature-sensitive mutant (NDVpi) isolated from persistently infected L cells. Less polymerase activity was associated with the NDVpi clones. Also, compared to NDVo, an increase in incubation temperature from 32 to 37 or 42 C resulted in a marked decrease in polymerase activity for the temperature-sensitive mutants which coincided with their inability to replicate at 42 C.

¹ Present address: Department of Pediatrics, Division of Infectious Diseases and Immunology, Emory University School of Medicine, 69 Butler Street, S.E., Atlanta, Ga. 30303.

² Present address: Department of Microbiology and Immunology, University of Oregon Health Science Center, Portland, Ore. 97201.