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In Vitro Transcription of 70S RNA by the RNA-Directed DNA Polymerase of Rous Sarcoma Virus: Lack of Influence of RNase H

Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455

ABSTRACT

The influence of Rous sarcoma virus (RSV)-associated RNase H on the in vitro synthesis of DNA by the RSV RNA-directed DNA polymerase was determined under conditions whereby RNase H activity was selectively inhibited with NaF. Not only were we unable to detect any effect on the size, structure, or genetic complexity of the DNA product synthesized in the absence of RNase H activity, but the displacement of DNA from the 70S RNA:DNA hybrid structures was also unaffected. The suitability of 70S RNA:DNA hybrid structures synthesized in vitro to serve as a substrate for RNase H is discussed.