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J Virol. 1976 January; 17(1): 287-290
Copyright © 1976 American Society for Microbiology. All Rights Reserved.

Rapid Purification of 70S RNA from Media of Cells Producing RNA Tumor Viruses

¹ Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, Maryland 20014

ABSTRACT

Media from cells producing RNA tumor viruses, when treated with sodium dodecyl sulfate and polyvinyl sulfate, yield 70S RNA as the major species binding oligo(dT)-cellulose. The procedure described for purifying 70S RNA requires no special equipment and is suitable for rapidly processing large quantities of media or for purifying RNA from commercially available virus, with a 5- to 10-fold higher yield than was obtained using existing methods.