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J Virol. 1976 January; 17(1): 254-268
Copyright © 1976 American Society for Microbiology. All Rights Reserved.

Replication of a Nuclear Polyhedrosis Virus in a Continuous Cell Culture of Spodoptera frugiperda: Microscopy Study of the Sequence of Events of the Virus Infection

^a N. E. R. C., Unit of Invertebrate Virology, Oxford OX1 3RB, England

ABSTRACT

A microscopy study of the sequence of morphogenic events of Spodoptera frugiperda nuclear polyhedrosis virus infection of S. frugiperda cells is presented which orders the sequence of replication and establishes the time scale within which the events occur. The virus entered the cell by 1 h postinfection and was uncoated. The eclipse period was 9 h and the latent period was 12 h. Polyhedron formation was detected by 18 h postinfection and continued until the deposition of the polyhedron membrane was completed by 48 h postinfection. Aberrant morphogenic characteristics of virus repeatedly passaged in the cell culture were also recorded. Adsorption, envelope morphogenesis, and release mechanisms are discussed in light of other data on in vivo and in vitro baculovirus infections.

¹ Present address: Department of Epidemiology and Public Health, Yale Arbovirus Research Unit, Yale University School of Medicine, New Haven, Conn. 06510.

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