This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Takemoto, K. K. Articles by Martin, M. A. Search for Related Content PubMed PubMed Citation Articles by Takemoto, K. K. Articles by Martin, M. A.

Transformation of Hamster Kidney Cells by BK Papovavirus DNA

¹ National Institute of Allergy and Infectious Diseases, Laboratory of Viral Diseases and Laboratory of Biology of Viruses, Bethesda, Maryland 20014

ABSTRACT

Supercoiled BK papovavirus DNA was shown to transform hamster kidney cells using the calcium phosphate co-precipitation technique. The transformed cells contained intranuclear T-antigen(s) and rescuable virus and produced progressively growing tumors when inoculated into hamsters. A novel finding was the production in tumor-bearing animals of antinuclear antibody, which reacted against normal, untransformed cells; in addition, tumor serum contained antibody against virus-specific T-antigen(s).